keyhole limpet

The following abbreviations are used in the examples: FPA: fibrinopeptide A ELISA: enzyme immunoassay (enzyme-linked immunosorbent assay) RIA: radioimmunoassay KLH: keyhole limpet hemocyanin PBS: phosphate-buffered saline Tris: tris(hydroxymethyl)aminomethane EDTA: ethylenedinitrilotetraacetic acid OD: absorbance (optical density) Asp: L-aspartic acid Ala: L-alanine Arg: L-arginine Gly: glycine Glu: L-glutamic acid Ile: L-isoleucine Ser: L-serine Cys(SH): L-cysteine Fmoc: 9-fluorenylmethyloxycarbonyl O-t-Bu: tert.-butyl ester t-Bu: tert.-butyl ether Mtr: 4-methoxy-2,3,6-trimethylphenylsulfonyl DMF: dimethylformamide RP-HPLC: reversed phase high performance liquid chromatography GMBS: gamma-maleimidobutyric acid hydroxysuccinimide ester EXAMPLE 1 Preparation of an Antigen for the Immunization a) Peptide synthesis for the pentadecapeptide H-Cys(SH)-Leu-Asp-Glu-Asp-Ser-Asp-Glu-Glu-Arg-Ala-Ile-Glu-Gly-Arg The peptide was synthesized using a semiautomatic peptide synthesizer.

In den Beispielen werden folgende Abkürzungen verwendet: FPA Fibrinopeptid A ELISA Enzymimmunoassay (enzyme linked immunosorbent assay) RIA Radioimmunoassay KLH Keyhole Limpet hemocyanin (Haemocyanin einer marinen Napfschnecke) PBS Phosphatgepufferte Kochsalzlösung (phosphate buffered saline) Tris Tris (hydroxymethyl) aminomethan EDTA Äthylendinitriloteetraessigsäure OD Extinktion (optical density) Asp L-Asparaginsäure Ala L-Alanin Arg L-Arginin Gly Glycin Glu L-Glutaminsäure Ile L-Isoleucin

A peptide of Claim 4 wherein the carrier protein is serum albumin, keyhole limpet hemocyanin diphtheria toxid or a synthetic polyaminoacid.

Ein Peptid gemäß Anspruch 4, worin das Trägerprotein Serumalbumin, Hämocyanin der Keyhole-Napfschnecke, Diphtherietoxoid oder ein synthetisches Polyaminosäure.